Anticorps Polyclonal de lapin anti-MAP7D1

MAP7D1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20788-1-PBS

Synonymes

Arginine/proline-rich coiled-coil domain-containing protein 1, KIAA1187, MAP7 domain-containing protein 1, PARCC1, PP2464

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Galerie de données de validation

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  WB analysis using 20788-1-AP (same clone as 20788-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20788-1-AP (MAP7D1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20788-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 20788-1-AP (same clone as 20788-1-PBS)

  IP result of anti-MAP7D1 (IP:20788-1-AP, 4ug; Detection:20788-1-AP 1:30000) with HEK-293 cells lysate 1470 ug. This data was developed using the same antibody clone with 20788-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 20788-1-AP (same clone as 20788-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20788-1-AP (MAP7D1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20788-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 20788-1-AP (same clone as 20788-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20788-1-AP (MAP7D1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20788-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 20788-1-AP (same clone as 20788-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using MAP7D1 antibody (20788-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20788-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 20788-1-AP (same clone as 20788-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAP7D1 antibody (20788-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20788-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20788-1-PBS cible MAP7D1 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAP7D1 Protéine recombinante Ag14385
• Nom complet: MAP7 domain containing 1
• Masse moléculaire calculée: 841 aa, 93 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: BC003083
• Symbole du gène: MAP7D1
• Identification du gène (NCBI): 55700
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MAP7D1 also known as RPRC1, PARCC1, belongs to the MAP7 family. The MAP7 (Microtubule Associated Protein 7) protein family, consisting of four members, MAP7, MAP7D1, and MAP7D2, MAP7D3, is the microtubule-associated protein involved in various cellular processes regulating microtubule dynamics, organization, and stability(PMID: 28980356). MAP7D1 exhibits the highest conservation with MAP7 and was recently identified as a phosphorylation substrate of DCLK1 in cortical neurons. MAP7D1 is required to maintain MT acetylation, which is enriched in stable MTs(PMID: 35470240). Consistent with the literature, the apparent molecular mass of MAP7D1 detected by Western blot was 120-130 kDa (PMID: 35470240, 37550720).

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