Anticorps Recombinant de lapin anti-MAP7D1

MAP7D1 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230424A6

N° de cat : 83173-1-PBS

Synonymes

230424A6, Arginine/proline-rich coiled-coil domain-containing protein 1, KIAA1187, MAP7 domain-containing protein 1, PARCC1

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Galerie de données de validation

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  WB analysis using 83173-1-RR (same clone as 83173-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83173-1-RR (MAP7D1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83173-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 83173-1-RR (same clone as 83173-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MAP7D1 antibody (83173-1-RR, Clone: 230424A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83173-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00212-2

  Cytometric bead array standard curve of MP00212-2, MAP7D1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83173-1-PBS. Detection antibody: 83173-3-PBS. Standard: Ag14385. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 83173-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83173-1-RR against Human MAP7D1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83173-1-PBS cible MAP7D1 dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MAP7D1 Protéine recombinante Ag14385
• Nom complet: MAP7 domain containing 1
• Masse moléculaire calculée: 841 aa, 93 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: BC003083
• Symbole du gène: MAP7D1
• Identification du gène (NCBI): 55700
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MAP7D1 also known as RPRC1, PARCC1, belongs to the MAP7 family. The MAP7 (Microtubule Associated Protein 7) protein family, consisting of four members, MAP7, MAP7D1, and MAP7D2, MAP7D3, is the microtubule-associated protein involved in various cellular processes regulating microtubule dynamics, organization, and stability(PMID: 28980356). MAP7D1 exhibits the highest conservation with MAP7 and was recently identified as a phosphorylation substrate of DCLK1 in cortical neurons. MAP7D1 is required to maintain MT acetylation, which is enriched in stable MTs(PMID: 35470240). Consistent with the literature, the apparent molecular mass of MAP7D1 detected by Western blot was 120-130 kDa (PMID: 35470240, 37550720).

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