Anticorps Recombinant de lapin anti-MAP7D1

MAP7D1 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

230424A6

N° de cat : 83173-1-RR

Synonymes

PP2464, PARCC1, MAP7 domain-containing protein 1, KIAA1187, Arginine/proline-rich coiled-coil domain-containing protein 1

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Galerie de données de validation

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  WB analysis using 83173-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83173-1-RR (MAP7D1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of U-251 using 83173-1-RR

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MAP7D1 antibody (83173-1-RR, Clone: 230424A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 83173-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83173-1-RR against Human MAP7D1 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MDA-MB-231
• Résultats positifs en IF/ICC: cellules U-251,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83173-1-RR cible MAP7D1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MAP7D1 Protéine recombinante Ag14385
• Nom complet: MAP7 domain containing 1
• Masse moléculaire calculée: 841 aa, 93 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: BC003083
• Symbole du gène: MAP7D1
• Identification du gène (NCBI): 55700
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAP7D1 also known as RPRC1, PARCC1, belongs to the MAP7 family. The MAP7 (Microtubule Associated Protein 7) protein family, consisting of four members, MAP7, MAP7D1, and MAP7D2, MAP7D3, is the microtubule-associated protein involved in various cellular processes regulating microtubule dynamics, organization, and stability(PMID: 28980356). MAP7D1 exhibits the highest conservation with MAP7 and was recently identified as a phosphorylation substrate of DCLK1 in cortical neurons. MAP7D1 is required to maintain MT acetylation, which is enriched in stable MTs(PMID: 35470240). Consistent with the literature, the apparent molecular mass of MAP7D1 detected by Western blot was 120-130 kDa (PMID: 35470240, 37550720).

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