Anticorps Polyclonal de lapin anti-JNK

• Phare
• Validé par KD/KO

JNK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(31)

N° de cat : 17572-1-AP

Synonymes

c Jun N terminal kinase 3, JNK, JNK3, JNK3A, MAP kinase 10, MAP kinase p49 3F12, MAPK 10, MAPK10, p493F12, p54bSAPK, PRKM10

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Galerie de données de validation

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  WB analysis using 17572-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17572-1-AP (MAPK10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 17572-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17572-1-AP (MAPK10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17572-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17572-1-AP (MAPK10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 17572-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using JNK antibody (17572-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu de cervelet de souris
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 31 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

17572-1-AP cible JNK dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: JNK Protéine recombinante Ag11785
• Nom complet: mitogen-activated protein kinase 10
• Masse moléculaire calculée: 464 aa, 53 kDa
• Poids moléculaire observé: 45-48 kDa, 54-57 kDa
• Numéro d’acquisition GenBank: BC065516
• Symbole du gène: JNK3
• Identification du gène (NCBI): 5602
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

JNK, also named MAPK10 (mitogen-activated protein kinase 10), belongs to MAP kinase family. MAP kinases act as integration points for multiple biochemical signals, and thus are involved in a wide variety of cellular processes, such as proliferation, differentiation, transcription regulation and development. This kinase is mainly expressed in a subset of neurons in the nervous system, and is activated by threonine and tyrosine phosphorylation. JNK has 3 isoforms of 48-53 kDa.

Publications

Species	Application	Title
	WB	Nat Commun; Protective effects of Pt-N-C single-atom nanozymes against myocardial ischemia-reperfusion injury; Authors - Tianbao Ye
rat	WB	J Neuroinflammation; SPOCK2 modulates neuropathic pain by interacting with MT1-MMP to regulate astrocytic MMP-2 activation in rats with chronic constriction injury; Authors - Chenglong Wang
mouse	WB	Int J Mol Sci; Sasanquasaponin from Camellia oleifera Abel Exerts an Anti-Inflammatory Effect in RAW 264.7 Cells via Inhibition of the NF-κB/MAPK Signaling Pathways; Authors - Yaxin Zhao
human,mouse	WB	Chem Biol Interact; Pulsatilla saponin D inhibited the growth of osteosarcoma by regulating the JNK/ATF3 signaling pathway; Authors - Kaipeng Jin
human	WB	Biomed Pharmacother; Icariside II suppresses cervical cancer cell migration through JNK modulated matrix metalloproteinase-2/9 inhibition in vitro and in vivo.; Authors - Ya-Sai Sun
human	WB,IHC	Onco Targets Ther; miR-137-3p Modulates the Progression of Prostate Cancer by Regulating the JNK3/EZH2 Axis.; Authors - Yachen Zang ; KD Validated

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