Anticorps Recombinant de lapin anti-JNK

JNK Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242364A10

N° de cat : 84875-5-PBS

Synonymes

MAPK10, 242364A10, c-Jun N-terminal kinase 3, EC:2.7.11.24, JNK1

---

Galerie de données de validation

• 

  IHC staining of mouse brain using 84875-5-RR (same clone as 84875-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84875-5-RR (MAPK10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84875-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 84875-5-RR (same clone as 84875-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84875-5-RR (MAPK10 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84875-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 84875-5-RR (same clone as 84875-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using JNK antibody (84875-5-RR, Clone: 242364A10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84875-5-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 84875-5-RR (same clone as 84875-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MAPK10 Recombinant antibody (84875-5-RR, Clone:242364A10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84875-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84875-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84875-5-RR against Human MAPK10 were performed. The affinity constant is 2.45 nM.

---

Informations sur le produit

84875-5-PBS cible JNK dans les applications de IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: JNK Protéine recombinante Ag11785
• Nom complet: mitogen-activated protein kinase 10
• Masse moléculaire calculée: 464 aa, 53 kDa
• Numéro d’acquisition GenBank: BC065516
• Symbole du gène: JNK3
• Identification du gène (NCBI): 5602
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

{{ptg:RelatedPrimaryAntibodies}}