Anticorps Polyclonal de lapin anti-MAPK11

• Phare
• Validé par KD/KO

MAPK11 Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 17376-1-AP

Synonymes

MAP kinase 11, MAP kinase p38 beta, MAPK 11, MAPK11, p38 2, P38B, p38Beta, P38BETA2, PRKM11, SAPK2, SAPK2B

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Galerie de données de validation

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  WB analysis using 17376-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17376-1-AP (MAPK11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 17376-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 17376-1-AP (MAPK11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 17376-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17376-1-AP (MAPK11 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 17376-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17376-1-AP (MAPK11 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 17376-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17376-1-AP (MAPK11 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HEK-293 using 17376-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 17376-1-AP (MAPK11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, tissu cardiaque humain, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

17376-1-AP cible MAPK11 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAPK11 Protéine recombinante Ag11184
• Nom complet: mitogen-activated protein kinase 11
• Masse moléculaire calculée: 364 aa, 41 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC027933
• Symbole du gène: MAPK11
• Identification du gène (NCBI): 5600
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAPK11(Mitogen-activated protein kinase 11) is also named as PRKM11, SAPK2, SAPK2B,p38b and belongs to the MAP kinase subfamily.It is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors.In mouse brain, both p38a and p38b are present in neurons, while p38b is also expressed in glial cells(PMID:15748168).

Publications

Species	Application	Title
mouse	WB	Theranostics; Interleukin 33-mediated inhibition of A-type K+ channels induces sensory neuronal hyperexcitability and nociceptive behaviors in mice.; Authors - Yiru Wang
human	WB	Mol Pharmacol; p38β MAPK signaling mediates exenatide-stimulated microglial β-endorphin expression.; Authors - Hai-Yun Wu ; KD Validated
human	WB,IHC	Tumour Biol; Clinicopathological significance of p38β, p38γ, and p38δ and its biological roles in esophageal squamous cell carcinoma.; Authors - Shutao Zheng ; KD Validated
rat	WB	BMC Endocr Disord; The role of MAPK11/12/13/14 (p38 MAPK) protein in dopamine agonist-resistant prolactinomas.; Authors - Shuman Wang ; KD Validated
	WB	J Hazard Mater; Metastatic effects of environmental carcinogens mediated by MAPK and UPR pathways with an in vivo Drosophila Model; Authors - Fangnon Firmin Fangninou