Anticorps Polyclonal de lapin anti-MAPK12

• Phare
• Validé par KD/KO

MAPK12 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 20184-1-AP

Synonymes

MAP kinase 12, Extracellular signal-regulated kinase 6, ERK-6, ERK6, ERK 6

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Galerie de données de validation

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  WB analysis using 20184-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20184-1-AP (MAPK12 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 20184-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 20184-1-AP (MAPK12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 20184-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20184-1-AP (MAPK12 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 20184-1-AP

  IP result of anti-MAPK12 (IP:20184-1-AP, 4ug; Detection:20184-1-AP 1:800) with mouse skeletal muscle tissue lysate 2200ug.

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  IHC staining of mouse heart using 20184-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20184-1-AP (MAPK12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 20184-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20184-1-AP (MAPK12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 20184-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20184-1-AP (MAPK12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 20184-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20184-1-AP (MAPK12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque de rat, tissu cardiaque humain, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu cardiaque de souris, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 4 publications below

Informations sur le produit

20184-1-AP cible MAPK12 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAPK12 Protéine recombinante Ag14073
• Nom complet: mitogen-activated protein kinase 12
• Masse moléculaire calculée: 367 aa, 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC015741
• Symbole du gène: MAPK12
• Identification du gène (NCBI): 6300
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IHC	Inflamm Regen; SPARC activates p38γ signaling to promote PFKFB3 protein stabilization and contributes to keloid fibroblast glycolysis; Authors - Yining Liu
human	WB,IHC	Tumour Biol; Clinicopathological significance of p38β, p38γ, and p38δ and its biological roles in esophageal squamous cell carcinoma.; Authors - Shutao Zheng ; KD Validated
rat	WB	BMC Endocr Disord; The role of MAPK11/12/13/14 (p38 MAPK) protein in dopamine agonist-resistant prolactinomas.; Authors - Shuman Wang
human	IHC	Clin Transl Oncol; A retrospective analysis of the clinicopathological features and prognostic value of MAPK12 protein expression in diffuse large B-cell lymphoma; Authors - Yue Liu
human,mouse	WB,IHC	J Orthop Translat; Targeting the senescence-related genes MAPK12 and FOS to alleviate osteoarthritis; Authors - Nana Geng
mouse	WB	Nat Microbiol; ERK pathway reactivation prevents anthrax toxin lethality in mice; Authors - Jie Liu

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