Anticorps Polyclonal de lapin anti-JNK

JNK Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 17337-1-AP

Synonymes

MAPK9, JNK2A, JNK2, JNK 55, EC:2.7.11.24

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Galerie de données de validation

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  WB analysis of mouse brain using 17337-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17337-1-AP (JNK Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of SH-SY5Y using 17337-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 17337-1-AP (JNK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IF: See 1 publications below

Informations sur le produit

17337-1-AP cible JNK dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: JNK Protéine recombinante Ag11372
• Nom complet: mitogen-activated protein kinase 9
• Masse moléculaire calculée: 424 aa, 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC032539
• Symbole du gène: JNK
• Identification du gène (NCBI): 5601
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

c-Jun N-terminal kinases (JNKs), first characterized as stress-activated members of the mitogen-activated protein kinase (MAPK) family, have shown their critical roles in the development of a number of cellular progress and diseases, including proliferation, embryonic development, apoptosis, diabetes, neurodegeneration and liver disease (PMID: 19900593). JNK1, JNK2, JNK3 also name as MAPK8, MAPK9, MAPK10, JNK1 and JNK2 are ubiquitously expressed, but JNK3 is expressed primarily in the brain (PMID: 27698029). This antibody can recognize JNK1, JNK2 and JNK3, we get bands around 44-55 kDa.

Publications

Species	Application	Title
human	WB	Biomed Pharmacother; Molecular mechanism through which Tripterygium hypoglaucum (Lévl.) Hutch alleviates psoriasis; Authors - Yumei Sun
mouse	WB	Int Immunopharmacol; RG108 attenuates acute kidney injury by inhibiting P38 MAPK/FOS and JNK/JUN pathways; Authors - Fan-Xu Kong
mouse	WB	Adv Sci (Weinh); Ultra-Long-Term Anti-Inflammatory Polyphenol Capsule to Remodel the Microenvironment for Accelerating Osteoarthritis Healing by Single Dosage; Authors - Shaoyin Wei
human	WB	BMC Cancer; Aldo-keto reductase family 1 member C3 mediates radioresistance of esophageal cancer cells through suppressing MAPK and AKT signaling; Authors - Wei Xiong
mouse	WB	Cardiovasc Diabetol; ANXA1-FPR2 axis mitigates the susceptibility to atrial fibrillation in obesity via rescuing AMPK activity in response to lipid overload; Authors - Peng Liu
rat	WB	Int Immunopharmacol; Kaempferol attenuates experimental autoimmune neuritis through TNFR1/JNK/p38 signaling pathway inhibition; Authors - Li Wang

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