Anticorps Polyclonal de lapin anti-JNK

JNK Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, singe, souris et plus (2)

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(22)

N° de cat : 51153-1-AP

Synonymes

MAPK9, c Jun N terminal kinase 2, c-Jun N-terminal kinase 2, EC:2.7.11.24, JNK 55

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Galerie de données de validation

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  WB analysis of HeLa using 51153-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51153-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 51153-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 51153-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW264.7 using 51153-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 51153-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 51153-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 51153-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COS-7 using 51153-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 51153-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 51153-1-AP

  IP result of anti-JNK (IP:51153-1-AP, 4ug; Detection:51153-1-AP 1:5000) with HeLa cells lysate 1280 ug.

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  IHC staining of human intrahepatic cholangiocarcinoma using 51153-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 51153-1-AP (JNK antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 51153-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 51153-1-AP (JNK antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HUVEC using 51153-1-AP

  Immunofluorescent analysis of HUVEC cells using 51153-1-AP (JNK antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules COS-7, cellules NIH/3T3
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 20 publications below
• IHC: See 3 publications below

Informations sur le produit

51153-1-AP cible JNK dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, singe, souris

• Réactivité: Humain, rat, singe, souris
• Réactivité citée: rat, bovin, Humain, Lapin, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: JNK Protéine recombinante Ag1442
• Nom complet: mitogen-activated protein kinase 9
• Masse moléculaire calculée: 424 aa, 48 kDa
• Poids moléculaire observé: 44-55 kDa
• Numéro d’acquisition GenBank: BC032539
• Symbole du gène: JNK
• Identification du gène (NCBI): 5601
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

c-Jun N-terminal kinases (JNKs), first characterized as stress-activated members of the mitogen-activated protein kinase (MAPK) family, have shown their critical roles in the development of a number of cellular progress and diseases, including proliferation, embryonic development, apoptosis, diabetes, neurodegeneration and liver disease (PMID: 19900593). JNK1, JNK2, JNK3 also name as MAPK8, MAPK9, MAPK10, JNK1 and JNK2 are ubiquitously expressed, but JNK3 is expressed primarily in the brain (PMID: 27698029). This antibody can recognize JNK1, JNK2 and JNK3, we get bands around 44-55 kDa.

Publications

Species	Application	Title
human	WB	Adv Mater; m6 A Reader YTHDF1-Targeting Engineered Small Extracellular Vesicles for Gastric Cancer Therapy via Epigenetic and Immune Regulation; Authors - Qing You
human	WB	Cell Rep Med; Development of an orally bioavailable CDK12/13 degrader and induction of synthetic lethality with AKT pathway inhibition; Authors - Yu Chang
human	WB	Nat Commun; SH3RF3 promotes breast cancer stem-like properties via JNK activation and PTX3 upregulation.; Authors - Peiyuan Zhang
mouse	WB	Int J Biol Macromol; Flammulina velutipes polysaccharide exerts immunomodulatory function involving RSAD2 to regulate the NF-κB/MAPK signaling pathway in RAW264.7 macrophage cells and in mouse spleen cells; Authors - Qiuyi Wang
human	WB	Biomed Pharmacother; Icariside II suppresses cervical cancer cell migration through JNK modulated matrix metalloproteinase-2/9 inhibition in vitro and in vivo.; Authors - Ya-Sai Sun
human	WB	Fitoterapia; The key active ingredients identification and pharmacological mechanism investigation of extract of ethyl acetate from Er Miao San aganist rheumatoid arthritis; Authors - Jia-Yu Wang

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