Anticorps Polyclonal de lapin anti-MAPKAPK2

MAPKAPK2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 13949-1-AP

Synonymes

MAPKAP kinase 2, MAPKAPK 2, MAPKAPK2, MK2

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Galerie de données de validation

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  WB analysis using 13949-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 13949-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 13949-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 13949-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 13949-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13949-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 13949-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 13949-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 13949-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13949-1-AP (MAPKAPK2 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L); F-actin is stained using CL555-phalloidin (red).

• 

  IF Staining of HeLa using 13949-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, tissu de côlon de souris, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 1 publications below

Informations sur le produit

13949-1-AP cible MAPKAPK2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAPKAPK2 Protéine recombinante Ag5060
• Nom complet: mitogen-activated protein kinase-activated protein kinase 2
• Masse moléculaire calculée: 400 aa, 46 kDa
• Poids moléculaire observé: 47-50 kDa
• Numéro d’acquisition GenBank: BC036060
• Symbole du gène: MAPKAPK2
• Identification du gène (NCBI): 9261
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAPKAPK2(mitogen-activated protein kinase-activated protein kinase 2) is also named as MK2, MAPKAP-K2, MK-2 and belongs to the CAMK Ser/Thr protein kinase family. MAPKAPK2, one of several kinases directly phosphorylated and activated by p38 MAPK, plays a central role in the inflammatory response and is in the nucleus of unstimulated cells and moves rapidly to the cytoplasm after stimulation(PMID:12171911). It is also involved in many other cellular processes including stress responses, nuclear export, gene expression regulation and cell proliferation. Multiple residues of MAPKAPK2 are generally phosphorylated in vivo in response to stress, but only 4 residues(Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in vitro(PMID:22351694). It has 2 isoforms produced by alternative splicing and the range of the molecular weight is 42-60 kDa according to the references(PMID:10666409; 11328854;8995385).

Publications

Species	Application	Title
human	WB	Mol Cancer Res; Sophoridine inhibits human colorectal cancer progression via targeting MAPKAPK2.; Authors - Rui Wang
mouse	WB	Food Funct; L-Theanine mediates the p38MAPK signaling pathway to alleviate heat-induced oxidative stress and inflammation in mice.; Authors - Kehong Liu
human	WB	Toxicol Appl Pharmacol; p38 MAPK-DRP1 signaling is involved in mitochondrial dysfunction and cell death in mutant A53T α-synuclein model of Parkinson's disease.; Authors - Chen Gui
human	WB	Int J Mol Med; Aryl hydrocarbon receptor inhibits inflammation in DSS‑induced colitis via the MK2/p‑MK2/TTP pathway.; Authors - Qimeng Wang
mouse	WB	Brain Res Bull; The p38 mitogen activated protein kinase regulates β-amyloid protein internalization through the α7 nicotinic acetylcholine receptor in mouse brain.; Authors - Kai-Ge Ma
mouse	WB	Cell Rep; MK2 promotes Tfcp2l1 degradation via β-TrCP ubiquitin ligase to regulate mouse embryonic stem cell self-renewal.; Authors - Yan Zhang