Anticorps Recombinant de lapin anti-MARCH1

MARCH1 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242143G10

N° de cat : 85308-2-PBS

Synonymes

MARCHF1, E3 ubiquitin-protein ligase MARCHF1, EC:2.3.2.27, MARCH I, MARCH-I

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Galerie de données de validation

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  WB analysis of Daudi using 85308-2-RR (same clone as 85308-2-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 85308-2-RR (MARCH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85308-2-PBS in a different storage buffer formulation.

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  WB analysis using 85308-2-RR (same clone as 85308-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85308-2-RR (MARCH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85308-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 85308-2-RR (same clone as 85308-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85308-2-RR (MARCH1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85308-2-PBS in a different storage buffer formulation.

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  IF Staining of HEL using 85308-2-RR (same clone as 85308-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEL cells using MARCH1 antibody (85308-2-RR, Clone: 242143G10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85308-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85308-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85308-2-RR against Human MARCH1 were performed. The affinity constant is 29.7 nM.

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Informations sur le produit

85308-2-PBS cible MARCH1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MARCH1 Protéine recombinante Ag20231
• Nom complet: membrane-associated ring finger (C3HC4) 1
• Masse moléculaire calculée: 289 aa, 32 kDa
• Poids moléculaire observé: 31-35 kDa
• Numéro d’acquisition GenBank: BC153124
• Symbole du gène: MARCHF1
• Identification du gène (NCBI): 55016
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MARCH1 is mainly found in secondary lymphoid organs, more specifically in the endocytic pathway of dendritic cells (DCs) and B cells (11-15). MARCH1 reduces the half-life of peptide/MHC II complexes by causing their redistribution from recycling endosomes to lysosomes. MARCH1 homodimerizes and also forms heterodimers with others family members (PMID: 22508929).

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