Anticorps Polyclonal de lapin anti-MARCH5

• Phare
• Validé par KD/KO

MARCH5 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12213-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 12213-1-AP (same clone as 12213-1-PBS)

  WB result of MARCH5 antibody (12213-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-MARCH5 transfected HeLa cells. This data was developed using the same antibody clone with 12213-1-PBS in a different storage buffer formulation.

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  WB analysis using 12213-1-AP (same clone as 12213-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12213-1-AP (MARCH5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12213-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 12213-1-AP (same clone as 12213-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 12213-1-AP (MARCH5 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12213-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 12213-1-AP (same clone as 12213-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 12213-1-AP (MARCH5 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12213-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12213-1-PBS cible MARCH5 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MARCH5 Protéine recombinante Ag2806
• Nom complet: membrane-associated ring finger (C3HC4) 5
• Masse moléculaire calculée: 278 aa, 31 kDa
• Poids moléculaire observé: 31 kDa, 65-70 kDa
• Numéro d’acquisition GenBank: BC015480
• Symbole du gène: MARCH5
• Identification du gène (NCBI): 54708
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MARCH5(E3 ubiquitin-protein ligase MARCH5) is a 31 kDa protein also named as RNF153. This protein plays a protective role against mitochondrial dysfunction caused by the mitochondrial accumulation of mSOD1 via the ubiquitin-proteasome pathway (PMID:19741096). And it may regulate MAP1B LC1 function in mitochondria by controlling mitochondrial aggregation via mitochondrial LC1 levels(PMID:22308378). MEARCH5 can exsit as a homodimer with MW 65-70 kDa.

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