Anticorps Polyclonal de lapin anti-MARCH8

• Phare
• Validé par KD/KO

MARCH8 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(30)

N° de cat : 14119-1-AP

Synonymes

MARCHF8, Membrane-associated RING finger protein 8, MARCH-VIII, EC:2.3.2.27, E3 ubiquitin-protein ligase MARCHF8

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Galerie de données de validation

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  WB analysis of mouse testis using 14119-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14119-1-AP (MARCH8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 14119-1-AP

  IP result of anti-MARCH8 (IP:14119-1-AP, 4ug; Detection:14119-1-AP 1:500) with mouse testis tissue lysate 1120 ug.

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 12 publications below
• WB: See 23 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 4 publications below
• CoIP: See 2 publications below

Informations sur le produit

14119-1-AP cible MARCH8 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MARCH8 Protéine recombinante Ag5268
• Nom complet: membrane-associated ring finger (C3HC4) 8
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 30-33 kDa
• Numéro d’acquisition GenBank: BC066988
• Symbole du gène: MARCH8
• Identification du gène (NCBI): 220972
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MARCH8 (Membrane-associated RING finger protein 8) is also named as MIR, RNF178 and belongs to the RING zinc finger protein family. It functions as an E3 ubiquitin ligase for immune recognition-related molecules (e.g. major histocompatibility complex class I, B7-2, and ICAM-1). It plays a key role in controlling MHC class II surface expression by regulated ubiquitination of a lysine residue in the beta-chain (PMID:22761441).

Publications

Species	Application	Title
human	WB	Mol Cell; PMID: 28965816; Authors - Shouheng Jin ; KO Validated
human	WB	Nat Commun; MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes.; Authors - Xiaoman Liu
		Autophagy; BST2 suppresses porcine epidemic diarrhea virus replication by targeting and degrading virus nucleocapsid protein with selective autophagy.; Authors - Ning Kong ; KD Validated
human	WB	Autophagy; 2AB protein of Senecavirus A antagonizes selective autophagy and type I interferon production by degrading LC3 and MARCHF8.; Authors - Dage Sun ; KD Validated
human	WB	Proc Natl Acad Sci U S A; The E3 ubiquitin ligase MARCH8 negatively regulates IL-1β-induced NF-κB activation by targeting the IL1RAP coreceptor for ubiquitination and degradation.; Authors - Chen Rui R
human	WB	Cancers (Basel); MARCH8 Suppresses Tumor Metastasis and Mediates Degradation of STAT3 and CD44 in Breast Cancer Cells.; Authors - Wenjing Chen

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