Anticorps Polyclonal de lapin anti-MARCKS

MARCKS Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 20661-1-AP

Synonymes

MARCS, MACS, 80K-L protein, 80KL, 80K L protein

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Galerie de données de validation

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  WB analysis using 20661-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20661-1-AP (MARCKS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20661-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20661-1-AP (MARCKS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 20661-1-AP

  IP result of anti-MARCKS (IP:20661-1-AP, 4ug; Detection:20661-1-AP 1:300) with A431 cells lysate 1200ug.

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  IHC staining of human normal colon using 20661-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20661-1-AP (MARCKS antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 20661-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MARCKS antibody (20661-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules A431
• Résultats positifs en IHC: human normal colon, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

20661-1-AP cible MARCKS dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: myristoylated alanine-rich protein kinase C substrate
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: NM_002356
• Symbole du gène: MARCKS
• Identification du gène (NCBI): 4082
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MARCKS, also named as MACS, PRKCSL and 80K-L protein, belongs to the MARCKS family. MARCKS is the most prominent cellular substrate for protein kinase C. It binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

Publications

Species	Application	Title
rat	WB	J Biol Chem; Oncoprotein DJ-1 interacts with mTOR complexes to effect transcription factor Hif1α-dependent expression of collagen I (α2) during renal fibrosis.; Authors - Falguni Das
human	WB	J Proteomics; Proteomic profile of human dental follicle stem cells and apical papilla stem cells.; Authors - Tong Lei
human	IP	Int J Oncol; Myristoylated alanine-rich C-kinase substrate effector domain phosphorylation regulates the growth and radiation sensitization of glioblastoma.; Authors - Nicholas J Eustace
human	WB,IF	Front Med (Lausanne); Enhanced understanding of cinnamaldehyde's therapeutic potential in osteoarthritis through bioinformatics and mechanistic validation of its anti-apoptotic effect; Authors - Yueyang Sheng
human	WB	J Vet Med Sci; A peptide against the N-terminus of myristoylated alanine-rich C kinase substrate promotes neuronal differentiation in SH-SY5Y human neuroblastoma cells; Authors - Jannatul Ferdous
mouse	WB	bioRxiv; Sex specific disruptions in Protein Kinase Cγ signaling in a mouse model of Spinocerebellar Ataxia Type 14; Authors - Sarah A Wolfe

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