Anticorps Recombinant de lapin anti-MARS

MARS Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240648G3

N° de cat : 83690-5-PBS

Synonymes

MARS1, METRS, Methionyl-tRNA synthetase, Methionine--tRNA ligase, cytoplasmic, EC:6.1.1.10

---

Galerie de données de validation

• 

  WB analysis using 83690-5-RR (same clone as 83690-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83690-5-RR (MARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83690-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 83690-5-RR (same clone as 83690-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83690-5-RR (MARS antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83690-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovarian cancer using 83690-5-RR (same clone as 83690-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83690-5-RR (MARS antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83690-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 83690-5-RR (same clone as 83690-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MARS antibody (83690-5-RR, Clone: 240648G3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83690-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83690-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83690-5-RR against Human MARS were performed. The affinity constant is 0.459 nM.

---

Informations sur le produit

83690-5-PBS cible MARS dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MARS Protéine recombinante Ag6619
• Nom complet: methionyl-tRNA synthetase
• Masse moléculaire calculée: 101 kDa
• Poids moléculaire observé: 101 kDa
• Numéro d’acquisition GenBank: BC002384
• Symbole du gène: MARS
• Identification du gène (NCBI): 4141
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The methionyl-tRNA synthetase (MARS) gene encodes cytoplasmic methionyl-tRNA synthetase (MetRS) responsible for catalyzing the ligation of methionine to tRNA. MetRS belongs to a family of aminoacyl-tRNA synthetases that play critical roles in protein biosynthesis by charging tRNAs with their cognate amino acids. MetRS overexpression was shown to be evident in human colon cancer patients. MetRS may thus be involved in oncogenic transformation. MetRS has been proven as a potential prognostic marker candidate for the clinical prognostic prediction of non-small-cell lung cancer (NSCLC) in patients. MetRS can also be used to detect intracellular oxidative stress and control protein synthesis under oxidative stress (PMID: 30271085, PMID: 34679529, PMID: 32404475). The calculated molecular weight of MetRS is 101 kDa.

{{ptg:RelatedPrimaryAntibodies}}