Anticorps Polyclonal de lapin anti-MAST1

• Phare
• Validé par KD/KO

MAST1 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 13305-1-AP

Synonymes

SAST170, SAST, Microtubule-associated serine/threonine-protein kinase 1, MAST 1, KIAA0973

---

Galerie de données de validation

• 

  WB analysis using 13305-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13305-1-AP (MAST1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13305-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13305-1-AP (MAST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 13305-1-AP

  IP result of anti-MAST1 (IP:13305-1-AP, 4ug; Detection:13305-1-AP 1:500) with mouse brain tissue lysate 4000ug.

---

Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

13305-1-AP cible MAST1 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAST1 Protéine recombinante Ag4126
• Nom complet: microtubule associated serine/threonine kinase 1
• Masse moléculaire calculée: 1570 aa, 171 kDa
• Poids moléculaire observé: 171 kDa
• Numéro d’acquisition GenBank: BC027985
• Symbole du gène: MAST1
• Identification du gène (NCBI): 22983
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAST1(microtubule associated serine/threonine kinase 1) is also named as KIAA0973, SAST(syntrophin-associated serine/threonine-protein kinase), SAST170 and belongs to the family of microtubule-associated serine/threonine kinases, which has a unique structural feature with a serine/threonine kinase domain and a postsynaptic density protein-95/discs large/zona occludens-1 domain(PMID:12614677). This protein plays a role in a broad range of neural functions and it appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins.

Publications

Species	Application	Title
mouse	WB	Neuron; Mutations in MAST1 Cause Mega-Corpus-Callosum Syndrome with Cerebellar Hypoplasia and Cortical Malformations.; Authors - Ratna Tripathy ; KO Validated
human	WB,IHC,IF,IP	Theranostics; CRISPR/Cas9-based genome-wide screening for deubiquitinase subfamily identifies USP1 regulating MAST1-driven cisplatin-resistance in cancer cells; Authors - Apoorvi Tyagi

{{ptg:RelatedPrimaryAntibodies}}