Anticorps Polyclonal de lapin anti-MAT2B

MAT2B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

21

N° de cat : 15952-1-AP

Synonymes

MAT II, MAT II beta, MAT2B, MATIIbeta, Nbla02999, SDR23E1, TGR, UNQ2435/PRO4995

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Galerie de données de validation

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  WB analysis of HepG2 using 15952-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15952-1-AP (MAT2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15952-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15952-1-AP (MAT2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15952-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15952-1-AP (MAT2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 15952-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15952-1-AP (MAT2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15952-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15952-1-AP (MAT2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15952-1-AP

  IP Result of anti-MAT2B (IP:15952-1-AP, 4ug; Detection:15952-1-AP 1:500) with HepG2 cells lysate 1000ug.

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  IHC staining of human lung using 15952-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15952-1-AP (MAT2B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 15952-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15952-1-AP (MAT2B antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse lung using 15952-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 15952-1-AP (MAT2B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 15952-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 15952-1-AP (MAT2B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 15952-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15952-1-AP (MAT2B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 15952-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15952-1-AP (MAT2B antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 15952-1-AP

  Immunofluorescent analysis of HepG2 cells, using MAT2B antibody 15952-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 15952-1-AP

  Immunofluorescent analysis of HepG2 cells, using MAT2B antibody 15952-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293, cellules Jurkat, tissu cérébral de souris, tissu testiculaire de souris
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu pulmonaire humain, tissu pulmonaire de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 19 publications below
• IHC: See 1 publications below

Informations sur le produit

15952-1-AP cible MAT2B dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAT2B Protéine recombinante Ag8649
• Nom complet: methionine adenosyltransferase II, beta
• Masse moléculaire calculée: 36 kDa, 38 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC005218
• Symbole du gène: MAT2B
• Identification du gène (NCBI): 27430
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa
human		Ann N Y Acad Sci; PMID: 29722030; Authors - Holly M Brown-Borg
rat	WB	J Agric Food Chem; Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.; Authors - Shu Yang
pig	WB	J Agric Food Chem; Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets.; Authors - Yimin Jia
chicken	WB	Sci Rep; In ovo injection of betaine alleviates corticosterone-induced fatty liver in chickens through epigenetic modifications.; Authors - Yun Hu
rat	WB	J Nutr Biochem; Maternal betaine supplementation attenuates glucocorticoid-induced hepatic lipid accumulation through epigenetic modification in adult offspring rats.; Authors - Nannan Zhao