Anticorps Monoclonal anti-MATR3

MATR3 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

3B12C7

N° de cat : 68280-1-Ig

Synonymes

Matrin-3, matrin 3, KIAA0723

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Galerie de données de validation

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  WB analysis using 68280-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68280-1-Ig (MATR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis using 68280-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68280-1-Ig (MATR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 68280-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MATR3 antibody (68280-1-Ig, Clone: 3B12C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules A431, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules K-562, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68280-1-Ig cible MATR3 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MATR3 Protéine recombinante Ag31502
• Nom complet: matrin 3
• Masse moléculaire calculée: 847 aa, 95 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC015031
• Symbole du gène: MATR3
• Identification du gène (NCBI): 9782
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MATR3, known as KIAA0723, is an 847 amino acid protein, which may play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. MATR3 was originally identified as a nuclear matrix protein containing both DNA and RNA binding domains (PMID: 2033075) with a wide variety of functions ranging from DNA damage response to transcriptional and post-transcriptional regulation of cellular and viral RNAs(PMID: 21771346). the calculated molecular weight of MATR3 is 94 kDa, but we detect a 125 kDa protein as a result of PMID: 28977530.

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