Anticorps Monoclonal anti-MAVS; VISA

• Phare
• Validé par KD/KO

MAVS; VISA Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(15)

CloneNo.

1A8E9

N° de cat : 66911-1-Ig

Synonymes

MAVS, 1A8E9, Cardif, IPS 1, IPS1

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Galerie de données de validation

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  WB analysis of A431 using 66911-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66911-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66911-1-Ig

  WB result of MAVS; VISA antibody (66911-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAVS; VISA transfected HeLa cells.

• 

  WB analysis using 66911-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66911-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66911-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 66911-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66911-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:1100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66911-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:1100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules L02
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:550-1:2200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 13 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

66911-1-Ig cible MAVS; VISA dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc, singe, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MAVS; VISA Protéine recombinante Ag5949
• Nom complet: mitochondrial antiviral signaling protein
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 50-55 kDa, 70-75 kDa
• Numéro d’acquisition GenBank: BC044952
• Symbole du gène: MAVS
• Identification du gène (NCBI): 57506
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN beta promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.

Publications

Species	Application	Title
	IF	Mol Cell; An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation; Authors - Stephanie Pei Tung Yiu
mouse,human	WB	J Immunother Cancer; Targeting KDM4C enhances CD8+ T cell mediated antitumor immunity by activating chemokine CXCL10 transcription in lung cancer.; Authors - Xiaohua Jie
human	WB	Front Immunol; Swine Acute Diarrhea Syndrome Coronavirus Nucleocapsid Protein Antagonizes Interferon-β Production via Blocking the Interaction Between TRAF3 and TBK1.; Authors - Zhihai Zhou
human	WB	J Virol; Encephalomyocarditis virus abrogates the IFN-β signaling pathway via its structural protein VP2.; Authors - Yumei Han
human	WB	Vet Microbiol; DDX56 antagonizes IFN-β production to enhance EMCV replication by inhibiting IRF3 nuclear translocation; Authors - Shujuan Xu
human	WB,IF	Virus Res; Host protein, HSP90β, antagonizes IFN-β signaling pathway and facilitates the proliferation of encephalomyocarditis virus in vitro.; Authors - Qian Li

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