Anticorps Polyclonal de lapin anti-MAVS; VISA

• Phare
• Validé par KD/KO

MAVS; VISA Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

49

Avis (1)

N° de cat : 14341-1-AP

Synonymes

CARDIF, IPS 1, IPS1, KIAA1271, MAVS, MAVS; VISA, VISA

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Galerie de données de validation

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  WB analysis using 14341-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14341-1-AP (MAVS; VISA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14341-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14341-1-AP (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HuH-7 using 14341-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 14341-1-AP (MAVS; VISA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14341-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14341-1-AP (MAVS; VISA Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14341-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14341-1-AP (MAVS; VISA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 14341-1-AP

  IP Result of anti-MAVS; VISA (IP:14341-1-AP, 3ug; Detection:14341-1-AP 1:1000) with HEK-293 cells lysate 1700ug.

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  IHC staining of human breast cancer using 14341-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14341-1-AP (MAVS; VISA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14341-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14341-1-AP (MAVS; VISA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 14341-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 14341-1-AP (MAVS; VISA Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 14341-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 14341-1-AP (MAVS; VISA Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 14341-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 14341-1-AP (MAVS; VISA antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 14341-1-AP

  Immunofluorescent analysis of Hela cells, using MAVS antibody 14341-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules HepG2, cellules HuH-7, cellules Jurkat
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cutané humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 13 publications below
• WB: See 55 publications below
• IHC: See 1 publications below
• IF: See 7 publications below
• IP: See 5 publications below
• CoIP: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

14341-1-AP cible MAVS; VISA dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc, singe
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAVS; VISA Protéine recombinante Ag5655
• Nom complet: mitochondrial antiviral signaling protein
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 50-55 kDa, 70-75 kDa
• Numéro d’acquisition GenBank: BC044952
• Symbole du gène: MAVS
• Identification du gène (NCBI): 57506
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN-β promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion; Authors - Wenwen Zheng
		Immunity; Decreased Expression of the Host Long-Noncoding RNA-GM Facilitates Viral Escape by Inhibiting the Kinase activity TBK1 via S-glutathionylation.; Authors - Yujia Wang
	WB	Mol Cell; An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation; Authors - Stephanie Pei Tung Yiu
human	WB	Nat Commun; MLL5 suppresses antiviral innate immune response by facilitating STUB1-mediated RIG-I degradation.; Authors - Peipei Zhou
human	WB	EMBO J; CircPVT1 promotes ER-positive breast tumorigenesis and drug resistance by targeting ESR1 and MAVS; Authors - Jia Yi ; KD Validated
pig	WB	Autophagy; Induction of autophagy and suppression of type I IFN secretion by CSFV.; Authors - Baoming Xie

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Damien (Verified Customer) (09-03-2019)

No convince by this anti-MAVS antibody.In fact, in human cells MAVS protein is expressed in two differents forms : short-MAVS (55kDa) and full-length MAVS (72 kDa). So I have to observe two bands by WB. However, after using different conditions with this antibody according to the manufacturer's protocol I always observe a smear of protein, and one band at 72 kDa. The expected on around 55 kDa never appear. Note that my cells lysates were not degraded since the others proteins that I want to reveal were great.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HeLa

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