Anticorps Polyclonal de lapin anti-MAX

• Phare
• Validé par KD/KO

MAX Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 10426-1-AP

Synonymes

bHLHd4, bHLHd5, bHLHd6, bHLHd7, bHLHd8, MAX, MYC associated factor X, orf1, Protein max

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Galerie de données de validation

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  WB analysis of HEK-293 using 10426-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10426-1-AP (MAX Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10426-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10426-1-AP (MAX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 10426-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10426-1-AP (MAX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• ChIP: See 4 publications below

Informations sur le produit

10426-1-AP cible MAX dans les applications de WB, IHC, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAX Protéine recombinante Ag0680
• Nom complet: MYC associated factor X
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC003525
• Symbole du gène: MAX
• Identification du gène (NCBI): 4149
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Max, a member of MAX family, contains a basic helix-loop-helix (bHLH) domain, is a transcription regulator. MAX can form a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC-MAX complex is a transcriptional activator, while the MAD-MAX complex is a repressor. MAX could dimerizated with another bHLH protein to form a heterodimer, such MYC or MAD. Thus bands recognized by this antibody much larger than predicted.

Publications

Species	Application	Title
mouse	WB	Cancer Cell; MAX Functions as a Tumor Suppressor and Rewires Metabolism in Small Cell Lung Cancer.; Authors - Arnaud Augert ; KO Validated
human	WB	Sci Adv; A MYC inhibitor selectively alters the MYC and MAX cistromes and modulates the epigenomic landscape to regulate target gene expression.; Authors - Austin G Holmes
human	WB	Nat Commun; PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression.; Authors - Xianchun Lan
human	WB,ChIP	Elife; Loss of MGA repression mediated by an atypical polycomb complex promotes tumor progression and invasiveness.; Authors - Haritha Mathsyaraja
human	WB	Cell Commun Signal; Novel dual-targeting c-Myc inhibitor D347-2761 represses myeloma growth via blocking c-Myc/Max heterodimerization and disturbing its stability.; Authors - Ruosi Yao
human	chIP	J Biol Chem; Role for carbohydrate response element-binding protein (ChREBP) in high glucose-mediated repression of long noncoding RNA Tug1.; Authors - Jianyin Long