Anticorps Recombinant de lapin anti-MAX

MAX Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

230215E3

N° de cat : 82958-1-RR

Synonymes

bHLHd8, bHLHd7, bHLHd6, bHLHd5, bHLHd4

---

Galerie de données de validation

• 

  WB analysis using 82958-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82958-1-RR (MAX antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 82958-1-RR

  WB result of MAX antibody (82958-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAX transfected HEK-293 cells.

• 

  ChIP experiment of NCCIT using 82958-1-RR

  Chromatin was prepared from NCCIT cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of MAX (82958-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

• 

  Affinity and Kinetic Characterization of 82958-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82958-1-RR against Human MAX were performed. The affinity constant is 0.257 nM.

---

Applications testées

• Résultats positifs en WB: cellules PC-3,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82958-1-RR cible MAX dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MAX Protéine recombinante Ag0680
• Nom complet: MYC associated factor X
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC003525
• Symbole du gène: MAX
• Identification du gène (NCBI): 4149
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Max, a member of MAX family, contains a basic helix-loop-helix (bHLH) domain, is a transcription regulator. MAX can form a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC-MAX complex is a transcriptional activator, while the MAD-MAX complex is a repressor. MAX could dimerizated with another bHLH protein to form a heterodimer, such MYC or MAD. Thus bands recognized by this antibody much larger than predicted.

{{ptg:RelatedPrimaryAntibodies}}