Anticorps Recombinant de lapin anti-MAX

MAX Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230215D9

N° de cat : 82958-6-PBS

Synonymes

bHLHd4, bHLHd5, bHLHd6, bHLHd7, bHLHd8

---

Galerie de données de validation

• 

  WB analysis using 82958-6-RR (same clone as 82958-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82958-6-RR (MAX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82958-6-PBS in a different storage buffer formulation.

• 

  IF Staining of PC-3 using 82958-6-RR (same clone as 82958-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using MAX antibody (82958-6-RR, Clone: 230215D9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82958-6-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82958-6-RR

  Biolayer interferometry (BLl) kinetic assays of 82958-6-RR against Human MAX were performed. The affinity constant is 0.257 nM.

---

Informations sur le produit

82958-6-PBS cible MAX dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MAX Protéine recombinante Ag0680
• Nom complet: MYC associated factor X
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC003525
• Symbole du gène: MAX
• Identification du gène (NCBI): 4149
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Max, a member of MAX family, contains a basic helix-loop-helix (bHLH) domain, is a transcription regulator. MAX can form a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC-MAX complex is a transcriptional activator, while the MAD-MAX complex is a repressor. MAX could dimerizated with another bHLH protein to form a heterodimer, such MYC or MAD. Thus bands recognized by this antibody much larger than predicted.

{{ptg:RelatedPrimaryAntibodies}}