Anticorps Monoclonal anti-MAdCAM1

MAdCAM1 Monoclonal Antibody for IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2A12E8

N° de cat : 66594-1-PBS

Synonymes

hMAdCAM 1, MACAM1, MAdCAM 1, MAdCAM1

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Galerie de données de validation

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  IHC staining of human colon using 66594-1-Ig (same clone as 66594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66594-1-Ig (MAdCAM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66594-1-Ig (same clone as 66594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66594-1-Ig (MAdCAM1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 66594-1-Ig (same clone as 66594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66594-1-Ig (MADCAM1 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 66594-1-Ig (same clone as 66594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66594-1-Ig (MADCAM1 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66594-1-Ig (same clone as 66594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66594-1-Ig (MADCAM1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66594-1-Ig (same clone as 66594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66594-1-Ig (MADCAM1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66594-1-Ig (same clone as 66594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66594-1-Ig (MAdCAM1 antibody) at dilution of 1:500 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 66594-1-Ig (same clone as 66594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66594-1-Ig (MAdCAM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with . This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 66594-1-Ig (same clone as 66594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66594-1-Ig (MAdCAM1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with . This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66594-1-Ig (same clone as 66594-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using MAdCAM1 antibody (66594-1-Ig, Clone: 2A12E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66594-1-Ig (same clone as 66594-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using MAdCAM1 antibody (66594-1-Ig, Clone: 2A12E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66594-1-PBS cible MAdCAM1 dans les applications de IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MAdCAM1 Protéine recombinante Ag16184
• Nom complet: mucosal vascular addressin cell adhesion molecule 1
• Masse moléculaire calculée: 406 aa, 43 kDa
• Numéro d’acquisition GenBank: BC142629
• Symbole du gène: MADCAM1
• Identification du gène (NCBI): 8174
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Lymphocyte recirculation through different organs is thought to be regulated by adhesion molecules ("homing receptors") recognizing tissue-specific vascular addressins on endothelium (PMID: 7511642). The mucosal vascular addressin, MAdCAM1, is an immunoglobulin superfamily adhesion receptor for lymphocytes that is expressed by mucosal venules and helps direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria (PMID: 7687523). MAdCAM1 can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes.

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