Anticorps Polyclonal de lapin anti-MBD1

MBD1 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 29998-1-AP

Synonymes

MBD 1

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Galerie de données de validation

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  WB analysis using 29998-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29998-1-AP (MBD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 29998-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MBD1 antibody (29998-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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  IF Staining of MCF-7 using 29998-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MBD1 antibody (29998-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat
• Résultats positifs en IF/ICC: cellules HeLa, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

29998-1-AP cible MBD1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MBD1 Protéine recombinante Ag31424
• Nom complet: methyl-CpG binding domain protein 1
• Masse moléculaire calculée: 67KD
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: NM_015846
• Symbole du gène: MBD1
• Identification du gène (NCBI): 4152
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MBD1, also known as RFT, PCM1, CXXC3, is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). MBD1 is a transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. MBD1 acts as a transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. Isoform 1 and isoform 2 can repress transcription from unmethylated promoters (PMID: 12665582, 14610093, 16757475).

Publications

Species	Application	Title
human	WB	J Hematol Oncol; Novel potent molecular glue degraders against broad range of hematological cancer cell lines via multiple neosubstrates degradation; Authors - Pengyun Li

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