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Anticorps Polyclonal de lapin anti-MBD1

MBD1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29998-1-PBS

Synonymes

MBD 1

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using MBD1 Polyclonal antibody (29998-1-AP)

    WB analysis using 29998-1-AP (same clone as 29998-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29998-1-AP (MBD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29998-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MBD1 Polyclonal antibody (29998-1-AP)

    IF Staining of HeLa using 29998-1-AP (same clone as 29998-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MBD1 antibody (29998-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 29998-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using MBD1 Polyclonal antibody (29998-1-AP)

    IF Staining of MCF-7 using 29998-1-AP (same clone as 29998-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MBD1 antibody (29998-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 29998-1-PBS in a different storage buffer formulation.

Informations sur le produit

29998-1-PBS cible MBD1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène MBD1 Protéine recombinante Ag31424
Nom complet methyl-CpG binding domain protein 1
Masse moléculaire calculée67KD
Poids moléculaire observé70-80 kDa
Numéro d’acquisition GenBankNM_015846
Symbole du gène MBD1
Identification du gène (NCBI) 4152
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MBD1, also known as RFT, PCM1, CXXC3, is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). MBD1 is a transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. MBD1 acts as a transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. Isoform 1 and isoform 2 can repress transcription from unmethylated promoters (PMID: 12665582, 14610093, 16757475).

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