Anticorps Recombinant de lapin anti-MBD2

MBD2 Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250339G9

N° de cat : 86393-3-PBS

Synonymes

Demethylase, DMTase, Methyl-CpG-binding domain protein 2, Methyl-CpG-binding protein MBD2, NY CO 41

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Galerie de données de validation

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  WB analysis using 86393-3-RR (same clone as 86393-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86393-3-RR (MBD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86393-3-PBS in a different storage buffer formulation.

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Informations sur le produit

86393-3-PBS cible MBD2 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: methyl-CpG binding domain protein 2
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43-50 kDa
• Numéro d’acquisition GenBank: NM_003927
• Symbole du gène: MBD2
• Identification du gène (NCBI): 8932
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MBD2, also named as DMTase, binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. MBD2 binds hemi-methylated DNA as well. It recruits histone deacetylases and DNA methyltransferases. MBD2 acts as transcriptional repressor and plays a role in gene silencing (PMID:9774669). MBD2 belongs to a highly conserved member of the MBD protein family, is considered a reader of DNA methylation and drives the crosstalk between DNA methylation and histone deacetylation (PMID: 40089655). MBD2 drives breast cancer progression through the modulation of epithelial-to-mesenchymal transition (PMID: 38556549).

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