Anticorps Polyclonal de lapin anti-MBD3

• Phare
• Validé par KD/KO

MBD3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14258-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of Y79 using 14258-1-AP (same clone as 14258-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 14258-1-AP (same clone as 14258-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 14258-1-AP (same clone as 14258-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 14258-1-AP (same clone as 14258-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Y79 using 14258-1-AP (same clone as 14258-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 14258-1-AP (same clone as 14258-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14258-1-AP (same clone as 14258-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14258-1-AP (same clone as 14258-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 14258-1-AP (same clone as 14258-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 14258-1-AP (same clone as 14258-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Y79 using 14258-1-AP (same clone as 14258-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 14258-1-AP (same clone as 14258-1-PBS)

  IP result of anti-MBD3 (IP:14258-1-AP, 4ug; Detection:14258-1-AP 1:800) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 14258-1-AP (same clone as 14258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14258-1-AP (MBD3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 14258-1-AP (same clone as 14258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14258-1-AP (MBD3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14258-1-AP (same clone as 14258-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using MBD3 antibody 14258-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14258-1-AP (same clone as 14258-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using MBD3 antibody 14258-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 14258-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14258-1-PBS cible MBD3 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MBD3 Protéine recombinante Ag5531
• Nom complet: methyl-CpG binding domain protein 3
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC043619
• Symbole du gène: MBD3
• Identification du gène (NCBI): 53615
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MBD3 does not bind DNA by itself. It recruits histone deacetylases and DNA methyltransferases. MBD3 acts as transcriptional repressor and plays a role in gene silencing.(PMID:9774669) .

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