Anticorps Polyclonal de lapin anti-MBIP

• Phare
• Validé par KD/KO

MBIP Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 10685-1-AP

Synonymes

MBIP, MUK binding inhibitory protein

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Galerie de données de validation

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  WB analysis of HepG2 using 10685-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10685-1-AP (MBIP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10685-1-AP

  WB result of MBIP antibody (10685-1-AP; 1:600; room temperature for 1.5 hours) with sh-Control and sh-MBIP transfected HEK-293 cells.

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  WB analysis of HEK-293 using 10685-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10685-1-AP (MBIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10685-1-AP

  IP Result of anti-MBIP (IP:10685-1-AP, 4ug; Detection:10685-1-AP 1:300) with HEK-293 cells lysate 2800ug.

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  IHC staining of human testis using 10685-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10685-1-AP (MBIP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 10685-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10685-1-AP (MBIP Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 10685-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10685-1-AP (MBIP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 10685-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10685-1-AP (MBIP Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 10685-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10685-1-AP (MBIP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu testiculaire humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below

Informations sur le produit

10685-1-AP cible MBIP dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MBIP Protéine recombinante Ag1061
• Nom complet: MAP3K12 binding inhibitory protein 1
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC005197
• Symbole du gène: MBIP
• Identification du gène (NCBI): 51562
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MBIP (MAP3K12 binding inhibitory protein 1), also known as MAPK upstream kinase (MUK)-binding inhibitory protein, is a 344 amino acid nuclear and cytoplasmic protein that assists in the activation of the JNK pathway by inhibiting ZPK activity. Ubiquitously expressed, MBIP is found at high levels in lung and heart and is one component of the ADA2A-containing complex (ATAC) in conjunction with ADA3, GCN5, CDC101, TADA2L, WDR5, YEATS2, DR1 and CRP2BP.

Publications

Species	Application	Title
human	WB	Nature; Characterizing the cancer genome in lung adenocarcinoma.; Authors - Weir Barbara A BA ; KD Validated
HUMAN	WB	EMBO J; Distinct roles of GCN5/PCAF-mediated H3K9ac and CBP/p300-mediated H3K18/27ac in nuclear receptor transactivation.; Authors - Qihuang Jin
human	WB	Mol Cell Biol; The double-histone-acetyltransferase complex ATAC is essential for mammalian development.; Authors - Guelman Sebastián S
human	WB	Cell Signal; MBIP promotes ESCC metastasis by activating MAPK pathway; Authors - Yanchun Ma
human	WB	J Biol Chem; Endoplasmic reticulum stress-responsive transcription factor ATF6α directs recruitment of the Mediator of RNA polymerase II transcription and multiple histone acetyltransferase complexes.; Authors - Sela Dotan D
human	WB	J Biol Chem; Human ATAC Is a GCN5/PCAF-containing acetylase complex with a novel NC2-like histone fold module that interacts with the TATA-binding protein.; Authors - Yuan-Liang Wang