Anticorps Polyclonal de lapin anti-MBOAT1

MBOAT1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 25615-1-AP

Synonymes

LPEAT1, LPLAT 1, LPSAT, Lyso PS acyltransferase, MBOAT1, OACT1

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Galerie de données de validation

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  WB analysis using 25615-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25615-1-AP (MBOAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

25615-1-AP cible MBOAT1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MBOAT1 Protéine recombinante Ag22470
• Nom complet: membrane bound O-acyltransferase domain containing 1
• Masse moléculaire calculée: 495 aa, 57 kDa
• Poids moléculaire observé: 45-57 kDa
• Numéro d’acquisition GenBank: BC150652
• Symbole du gène: MBOAT1
• Identification du gène (NCBI): 154141
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MBOAT1 (membrane bound O-acyltransferase domain containing 1), also known as LPLAT or LPSAT. It is expected to be located in membrane and widely expressed in colon, small intestine. MBOAT1 belongs to the membrane-bound O- acetyltransferase superfamily. Its encoded transmembrane protein is an enzyme that transfers organic compounds, preferably oleoyl-CoA, to the hydroxyl group of protein target in the membrane. The translocation that destroys the gene may be related to short finger toe syndrome. At the same time, it may also play a role in neurite growth during neuronal differentiation (PMID: 18772128). Studies have determined that phospholipid modifying enzymes MBOAT1 and MBOAT2 are iron sag inhibitors. MBOAT1/2 can inhibit iron sag by reshaping the phospholipid profile of cells, and their iron sag monitoring function is independent of GPX4 or FSP1. MBOAT1 and MBOAT2 are up-regulated by sex hormone receptors, namely estrogen receptor (ER) and androgen receptor (AR), respectively (PMID: 37267948). The calculated molecular weight of MBOAT1 is 57 kDa. This antibody can recognize the 40 kDa isoform of MBOAT1.