Anticorps Recombinant de lapin anti-MBOAT7

MBOAT7 Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240505E8

N° de cat : 83546-3-PBS

Synonymes

h mboa 7, EC:2.3.1.-, Bladder and breast carcinoma-overexpressed gene 1 protein, BB1, 240505E8

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Galerie de données de validation

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  IHC staining of mouse testis using 83546-3-RR (same clone as 83546-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83546-3-RR (MBOAT7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83546-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 83546-3-RR (same clone as 83546-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83546-3-RR (MBOAT7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83546-3-PBS in a different storage buffer formulation.

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  IF Staining of HuH-7 using 83546-3-RR (same clone as 83546-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using MBOAT7 antibody (83546-3-RR, Clone: 240505E8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83546-3-PBS in a different storage buffer formulation.

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  IF Staining of HuH-7 using 83546-3-PBS

  Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using MBOAT7 antibody (83546-3-PBS, Clone: 240505E8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of A549 using 83546-3-RR (same clone as 83546-3-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug MBOAT7 Recombinant antibody (83546-3-RR, Clone:240505E8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83546-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83546-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83546-3-RR against Human MBOAT7 were performed. The affinity constant is 0.573 nM.

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Applications testées

• Résultats positifs en IF/ICC: cellules HuH-7,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83546-3-PBS cible MBOAT7 dans les applications de IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MBOAT7 Protéine recombinante Ag24466
• Nom complet: membrane bound O-acyltransferase domain containing 7
• Masse moléculaire calculée: 472 aa, 53 kDa
• Numéro d’acquisition GenBank: BC006309
• Symbole du gène: MBOAT7
• Identification du gène (NCBI): 79143
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MBOAT7 (membrane-bound O-acyltransferase domain containing 7), also known as LPIAT, LENG4, or BB1, belongs to the MBOAT superfamily. MBOAT7 acylates lyso-phosphatidylinositol (lyso-PI) with arachidonyl-CoA and is involved in phosphatidylinositol acyl-chain remodeling in the Lands cycle (PMID: 30959108; PMID: 35509994). MBOAT7 deficiency in humans leads to developmental disorders of the central nervous system, with intellectual disability, epilepsy, and autism spectrum disorder (PMID: 37316513). The calculated molecular weight of MBOAT7 is 53 kDa. This antibody can recognize the 45 kDa isoform of MBOAT7.

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