Anticorps Polyclonal de lapin anti-Myelin basic protein
Myelin basic protein Polyclonal Antibody for IF, IHC, WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
WB, IHC, IF, FC, ELISA
Conjugaison
Non conjugué
N° de cat : 10458-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | tissu cérébral de souris, tissu cérébral de rat |
| Résultats positifs en IHC | tissu cérébral de souris, il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
| Résultats positifs en IF | tissu cérébral de souris, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochimie (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 38 publications below |
| IHC | See 19 publications below |
| IF | See 48 publications below |
| FC | See 1 publications below |
Informations sur le produit
10458-1-AP cible Myelin basic protein dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
| Réactivité | Humain, rat, souris |
| Réactivité citée | rat, Humain, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | Myelin basic protein Protéine recombinante Ag0713 |
| Nom complet | myelin basic protein |
| Masse moléculaire calculée | 33 kDa |
| Poids moléculaire observé | 14-23 kDa |
| Numéro d’acquisition GenBank | BC008749 |
| Symbole du gène | MBP |
| Identification du gène (NCBI) | 4155 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
MBP belongs to the myelin basic protein family. The classic group of MBP isoforms (isoform 4-isoform 14) are the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. MBP has six isoforms. Catalog#10458-1-AP is capable of recognizing multiple isoforms of MBP.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for Myelin basic protein antibody 10458-1-AP | Download protocol |
| IHC protocol for Myelin basic protein antibody 10458-1-AP | Download protocol |
| IF protocol for Myelin basic protein antibody 10458-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
ACS Nano Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia | ||
ACS Nano Supramolecular Hydrogel Microspheres of Platelet-Derived Growth Factor Mimetic Peptide Promote Recovery from Spinal Cord Injury | ||
Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model | ||
Microbiome The microbiota-gut-brain axis participates in chronic cerebral hypoperfusion by disrupting the metabolism of short-chain fatty acids. | ||
Brain Sensory neurons have an axon initial segment that initiates spontaneous activity in neuropathic pain. | ||
Dev Cell Rewired glycosylation activity promotes scarless regeneration and functional recovery in spiny mice after complete spinal cord transection. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kenzo (Verified Customer) (08-16-2023) | This MBP antibody worked well and generated robust immunofluorescence signals on mouse optic nerve sections.
|
FH Rebecca (Verified Customer) (02-10-2022) | Western performed on Biorad MiniPROTEAN 4-15% TGX precast gels and Precision Plus All Blue Protein Ladder and 4x Laemmli Sample Buffer. Blocking performed for 1h at room temperature using 5%BLOTTO. Primary antibody dilution was performed overnight at 4°C in 5%BLOTTO/0.05%Tween. Secondary antibody was LiCOR IRDye® 680RD Donkey anti-Mouse IgG (H + L) at 1:20000 dilution in PBS-Tween-SDS as per manufacturers instructions for 2h at room temperature. Blot showed clear bands of the expected isoform sizes with very low-none background/off target binding.
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