Anticorps Polyclonal de lapin anti-MBP

MBP Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Conjugaison

Non conjugué

Publications(132)

Avis (3)

N° de cat : 10458-1-AP

Synonymes

myelin basic protein

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Galerie de données de validation

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  WB analysis using 10458-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10458-1-AP (Myelin basic protein antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 10458-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10458-1-AP (Myelin basic protein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10458-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10458-1-AP (Myelin basic protein antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 10458-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Myelin basic protein antibody (10458-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 10458-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using Myelin basic protein antibody (10458-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en IF-Fro: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 62 publications below
• IHC: See 23 publications below
• IF: See 82 publications below
• FC: See 1 publications below

Informations sur le produit

10458-1-AP cible MBP dans les applications de WB, IHC, IF-P, IF-Fro, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, Lapin, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MBP Protéine recombinante Ag0713
• Nom complet: myelin basic protein
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 14-23 kDa
• Numéro d’acquisition GenBank: BC008749
• Symbole du gène: Myelin basic protein
• Identification du gène (NCBI): 4155
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MBP belongs to the myelin basic protein family. The classic group of MBP isoforms (isoform 4-isoform 14) are the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. MBP has six isoforms. Catalog#10458-1-AP is capable of recognizing multiple isoforms of MBP.

Publications

Species	Application	Title
mouse	IF	ACS Nano; Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia; Authors - Shishi Shen
rat	IF	ACS Nano; Supramolecular Hydrogel Microspheres of Platelet-Derived Growth Factor Mimetic Peptide Promote Recovery from Spinal Cord Injury; Authors - Weidong Wu
human,mouse	IF	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
rat	IF	Microbiome; The microbiota-gut-brain axis participates in chronic cerebral hypoperfusion by disrupting the metabolism of short-chain fatty acids.; Authors - Weiping Xiao
mouse	IHC	Brain; Sensory neurons have an axon initial segment that initiates spontaneous activity in neuropathic pain.; Authors - Ana I Nascimento
mouse	IHC	J Pineal Res; Circadian misalignment impairs oligodendrocyte myelination via Bmal1 overexpression leading to anxiety and depression-like behaviors; Authors - Yao Zuo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Samantha (Verified Customer) (08-29-2025)

Used in a Western Blot and detected myelin in human dorsal root ganglia and bound to recombinant myelin protein

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 1000
• Cell Tissue Type: Human DRG tissue lysate, recombinant myelin protein

FH

Kenzo (Verified Customer) (08-16-2023)

This MBP antibody worked well and generated robust immunofluorescence signals on mouse optic nerve sections.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse optic nerve

FH

Rebecca (Verified Customer) (02-10-2022)

Western performed on Biorad MiniPROTEAN 4-15% TGX precast gels and Precision Plus All Blue Protein Ladder and 4x Laemmli Sample Buffer. Blocking performed for 1h at room temperature using 5%BLOTTO. Primary antibody dilution was performed overnight at 4°C in 5%BLOTTO/0.05%Tween. Secondary antibody was LiCOR IRDye® 680RD Donkey anti-Mouse IgG (H + L) at 1:20000 dilution in PBS-Tween-SDS as per manufacturers instructions for 2h at room temperature. Blot showed clear bands of the expected isoform sizes with very low-none background/off target binding.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Rat brain whole tissue lysate (50ug)

MBP Antibody Western Blot validation (1:5000 dilution) in Rat brain whole tissue lysate (50ug) (Cat no:10458-1-AP)

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