Anticorps Monoclonal anti-MBP tag

MBP tag Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Protéine recombinante

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

4C6H4

N° de cat : 66003-1-PBS

Synonymes

MBP, Maltose Binding Protein

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Galerie de données de validation

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  WB analysis of Recombinant protein using 66003-1-Ig (same clone as 66003-1-PBS)

  Recombinant protein were subjected to SDS PAGE followed by western blot with 66003-1-Ig (MBP tag antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66003-1-PBS in a different storage buffer formulation.

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  IP experiment of Recombinant protein using 66003-1-Ig (same clone as 66003-1-PBS)

  IP result of anti-MBP tag (IP:66003-1-Ig, 5ug; Detection:66003-1-Ig 1:20000) with Recombinant protein protein lysate 800ug. This data was developed using the same antibody clone with 66003-1-PBS in a different storage buffer formulation.

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  IF Staining of Transfected cells using 66003-1-Ig (same clone as 66003-1-PBS)

  IF result of MBP tag antibody (66003-1-Ig, 1:1,000) with MBP-Tagged protein.Courtesy of Neeraj Tiwari, PhD, Yale School of Medicine, Yale University. This data was developed using the same antibody clone with 66003-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66003-1-PBS cible MBP tag dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Protéine recombinante

• Réactivité: Protéine recombinante
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MBP tag Protéine recombinante Ag0942
• Nom complet: MBP tag
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 40 kDa
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein.

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