Anticorps Polyclonal de lapin anti-MCCC1

MCCC1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30081-1-PBS

Synonymes

MCCA, MCC B, EC:6.4.1.4, 3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha, 3-methylcrotonyl-CoA carboxylase biotin-containing subunit

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Galerie de données de validation

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  WB analysis using 30081-1-AP (same clone as 30081-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30081-1-AP (MCCC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30081-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 30081-1-AP (same clone as 30081-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30081-1-AP (MCCC1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30081-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 30081-1-AP (same clone as 30081-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30081-1-AP (MCCC1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30081-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 30081-1-AP (same clone as 30081-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30081-1-AP (MCCC1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30081-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 30081-1-AP (same clone as 30081-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MCCC1 antibody (30081-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 30081-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 30081-1-AP (same clone as 30081-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MCCC1 antibody (30081-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 30081-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30081-1-PBS cible MCCC1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MCCC1 Protéine recombinante Ag32459
• Nom complet: methylcrotonoyl-Coenzyme A carboxylase 1 (alpha)
• Masse moléculaire calculée: 76 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC004187
• Symbole du gène: MCCC1
• Identification du gène (NCBI): 56922
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MCCC1 is the large subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism.

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