Anticorps Polyclonal de lapin anti-MCL1

• Phare
• Validé par KD/KO

MCL1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16225-1-PBS

Synonymes

Bcl2-L-3, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, EAT, Induced myeloid leukemia cell differentiation protein Mcl-1

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Galerie de données de validation

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  WB analysis using 16225-1-AP (same clone as 16225-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16225-1-PBS in a different storage buffer formulation.

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  WB analysis using 16225-1-AP (same clone as 16225-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16225-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 16225-1-AP (same clone as 16225-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16225-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse spleen using 16225-1-AP (same clone as 16225-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16225-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 16225-1-AP (same clone as 16225-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16225-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 16225-1-AP (same clone as 16225-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16225-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 16225-1-AP (same clone as 16225-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16225-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 16225-1-AP (same clone as 16225-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16225-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16225-1-AP (same clone as 16225-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16225-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 16225-1-AP (same clone as 16225-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16225-1-PBS in a different storage buffer formulation.

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  IP experiment of Raji using 16225-1-AP (same clone as 16225-1-PBS)

  IP result of anti-MCL1 (IP:16225-1-AP, 3ug; Detection:16225-1-AP 1:1000) with Raji cells lysate 800ug. This data was developed using the same antibody clone with 16225-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 16225-1-AP (same clone as 16225-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16225-1-AP (MCL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16225-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 16225-1-AP (same clone as 16225-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16225-1-AP (MCL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16225-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 16225-1-AP (same clone as 16225-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MCL1 antibody (16225-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 16225-1-PBS in a different storage buffer formulation.

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  FC experiment of Ramos using 16225-1-AP (same clone as 16225-1-PBS)

  1X10^6 Ramos cells were intracellularly stained with 0.5 ug Anti-Human MCL1 (16225-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 16225-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16225-1-PBS cible MCL1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MCL1 Protéine recombinante Ag10397
• Nom complet: myeloid cell leukemia sequence 1 (BCL2-related)
• Masse moléculaire calculée: 350 aa, 37 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC107735
• Symbole du gène: MCL1
• Identification du gène (NCBI): 4170
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumour malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target. MCL-1 can be alternatively spliced into a long form (MCL-1L, 40 kDa) or a short form (MCL-1S, 30 kDa). (PMID: 15467463, PMID: 15147382)

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