Anticorps Recombinant de lapin anti-MCL1

• Phare
• Validé par KD/KO

MCL1 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C7

N° de cat : 82695-2-PBS

Synonymes

1C7, Bcl2-L-3, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, EAT

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Galerie de données de validation

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  WB analysis using 82695-2-RR (same clone as 82695-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82695-2-RR (MCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82695-2-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82695-2-RR (same clone as 82695-2-PBS)

  WB result of MCL1 antibody (82695-2-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCL1 transfected HeLa cells. This data was developed using the same antibody clone with 82695-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 82695-2-RR (same clone as 82695-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82695-2-RR (MCL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82695-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 82695-2-RR (same clone as 82695-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82695-2-RR (MCL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82695-2-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82695-2-RR (same clone as 82695-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82695-2-RR (MCL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82695-2-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82695-2-RR (same clone as 82695-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82695-2-RR (MCL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82695-2-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 82695-2-RR (same clone as 82695-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MCL1 antibody (82695-2-RR, Clone: 1C7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82695-2-PBS in a different storage buffer formulation.

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Informations sur le produit

82695-2-PBS cible MCL1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MCL1 Protéine recombinante Ag10397
• Nom complet: myeloid cell leukemia sequence 1 (BCL2-related)
• Masse moléculaire calculée: 350 aa, 37 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC107735
• Symbole du gène: MCL1
• Identification du gène (NCBI): 4170
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumour malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target. MCL-1 can be alternatively spliced into a long form (MCL-1L, 40 kDa) or a short form (MCL-1S, 30 kDa). (PMID: 15467463, PMID: 15147382)

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