Anticorps Polyclonal de lapin anti-MCM10

MCM10 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(16)

N° de cat : 12251-1-AP

Synonymes

CNA43, DNA43, HsMCM10, MCM10, PRO2249, Protein MCM10 homolog

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Galerie de données de validation

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  WB analysis of Ramos using 12251-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 12251-1-AP (MCM10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Ramos,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 13 publications below
• IF: See 1 publications below

Informations sur le produit

12251-1-AP cible MCM10 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MCM10 Protéine recombinante Ag2899
• Nom complet: minichromosome maintenance complex component 10
• Masse moléculaire calculée: 874 aa, 97 kDa
• Poids moléculaire observé: 97-105 kDa
• Numéro d’acquisition GenBank: BC009108
• Symbole du gène: MCM10
• Identification du gène (NCBI): 55388
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MCM10, also named as HsMCM10, belongs to the MCM10 family. It acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, it plays a role in preventing DNA damage during replication and is essential for the integrity of the RECQ4-MCM replicative helicase complex. It mediates RECQ4 association with MCM2-7 helicase complex during DNA replication.

Publications

Species	Application	Title
human		Nat Commun; DONSON and FANCM associate with different replisomes distinguished by replication timing and chromatin domain.; Authors - Jing Zhang
human	WB	Nat Commun; BRCA2 associates with MCM10 to suppress PRIMPOL-mediated repriming and single-stranded gap formation after DNA damage.; Authors - Zhihua Kang
human	WB	Cancer Discov; DDX5 regulates DNA replication and is required for cell proliferation in a subset of breast cancer cells.; Authors - Mazurek Anthony A
human	WB	EMBO J; MCM10 mediates RECQ4 association with MCM2-7 helicase complex during DNA replication.; Authors - Xu Xiaohua X
human		J Cell Biol; Regulation of ETAA1-mediated ATR activation couples DNA replication fidelity and genome stability.; Authors - Divya Achuthankutty
human	WB	Cell Rep; Impaired p32 Regulation Caused by the Lymphoma-Prone RECQ4 Mutation Drives Mitochondrial Dysfunction.; Authors - Jiin-Tarng Wang

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