Anticorps Polyclonal de lapin anti-MCM2

• Phare
• Validé par KD/KO

MCM2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

33

Avis (1)

N° de cat : 10513-1-AP

Synonymes

BM28, CCNL1, cdc19, CDCL1, D3S3194, KIAA0030, MCM2, MITOTIN, Nuclear protein BM28

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Galerie de données de validation

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  WB analysis using 10513-1-AP

  Various cells were subjected to SDS PAGE followed by western blot with 10513-1-AP (MCM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10513-1-AP

  WB result of MCM2 antibody (10513-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM2 transfected HEK-293 cells.

• 

  IP experiment of HEK-293 using 10513-1-AP

  IP Result of anti-MCM2 (IP:10513-1-AP, 4ug; Detection:10513-1-AP 1:800) with HEK-293 cells lysate 2800ug.

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  IHC staining of human cervical cancer using 10513-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10513-1-AP (MCM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10513-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10513-1-AP (MCM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10513-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10513-1-AP (MCM2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 10513-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10513-1-AP (MCM2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10513-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCM2 antibody (10513-1-AP) at dilution of 1:20 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of human gliomas using 10513-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using 10513-1-AP (MCM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of NIH/3T3 using 10513-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Human MCM2 (10513-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules K-562
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, tissu de gliome humain
• Résultats positifs en cytométrie: cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 25 publications below
• IHC: See 10 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

10513-1-AP cible MCM2 dans les applications de WB, RIP, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MCM2 Protéine recombinante Ag0798
• Nom complet: minichromosome maintenance complex component 2
• Masse moléculaire calculée: 102 kDa
• Poids moléculaire observé: 116-125 kDa
• Numéro d’acquisition GenBank: BC007670
• Symbole du gène: MCM2
• Identification du gène (NCBI): 4171
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The MCM2-7 complex forms the core of the replicative helicase which acts as the molecular motor that uses ATP binding and hydrolysis to fuel the unwinding of double-stranded DNA at the replication fork in eukaryotes. This complex is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. MCM2, also named CDCL1 and BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division.

Publications

Species	Application	Title
mouse	WB,IP	Nature; CDC7-independent G1/S transition revealed by targeted protein degradation.; Authors - Jan M Suski
human	WB	Nat Commun; DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation; Authors - Xiaohua Xu
human	IF	Nat Commun; Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes; Authors - Hana Polasek-Sedlackova
	WB	Cancer Res; LncRNA AGPG confers endocrine resistance in breast cancer by promoting E2F1 activity; Authors - Shiyi Yu
human	WB,RIP	Int J Biol Sci; ESRG is critical to maintain the cell survival and self-renewal/pluripotency of hPSCs by collaborating with MCM2 to suppress p53 pathway; Authors - Shasha Li
human	WB	Biomaterials; Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.; Authors - Hongyuan Song

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mandi (Verified Customer) (03-02-2020)

Biotinylated secondary and Alex Fluor needed for decent signal vs non-specific staining

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Rat brain