Anticorps Monoclonal anti-MCM2

• Phare
• Validé par KD/KO

MCM2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F4D10

N° de cat : 66204-1-PBS

Synonymes

1F4D10, BM28, CCNL1, CDCL1, EC:3.6.4.12

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Galerie de données de validation

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  WB analysis using 66204-1-Ig (same clone as 66204-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66204-1-Ig (same clone as 66204-1-PBS)

  WB result of MCM2 antibody (66204-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 66204-1-Ig (same clone as 66204-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 66204-1-Ig (same clone as 66204-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 66204-1-Ig (same clone as 66204-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66204-1-Ig (same clone as 66204-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

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  IHC staining of Human tonsillitis using 66204-1-Ig (same clone as 66204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66204-1-Ig (MCM2 antibody) at dilution of 1:2000 (under 20x lens). This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

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  IHC staining of Human tonsillitis using 66204-1-Ig (same clone as 66204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66204-1-Ig (MCM2 antibody) at dilution of 1:2000 (under 20x lens). This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66204-1-Ig (same clone as 66204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66204-1-Ig (MCM2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66204-1-Ig (same clone as 66204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66204-1-Ig (MCM2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66204-1-PBS cible MCM2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MCM2 Protéine recombinante Ag0798
• Nom complet: minichromosome maintenance complex component 2
• Masse moléculaire calculée: 102 kDa
• Poids moléculaire observé: 116-125 kDa
• Numéro d’acquisition GenBank: BC007670
• Symbole du gène: MCM2
• Identification du gène (NCBI): 4171
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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