Anticorps Recombinant de lapin anti-MCM3

• Phare
• Validé par KD/KO

MCM3 Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

242103B9

N° de cat : 84792-5-RR

Synonymes

242103B9, DNA polymerase alpha holoenzyme-associated protein P1, EC:3.6.4.12, P1 MCM3, p102

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Galerie de données de validation

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  WB analysis using 84792-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84792-5-RR (MCM3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 84792-5-RR

  WB result of MCM3 antibody (84792-5-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM3 transfected HeLa cells.

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  IHC staining of human colon cancer using 84792-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84792-5-RR (MCM3 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 84792-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84792-5-RR (MCM3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 84792-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84792-5-RR (MCM3 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 84792-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MCM3 Recombinant antibody (84792-5-RR, Clone:242103B9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of K-562 using 84792-5-RR

  1x10^6 K-562 cells were intracellularly stained with 0.25 ug MCM3 Recombinant antibody (84792-5-RR, Clone:242103B9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 84792-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84792-5-RR against Human MCM3 were performed. The affinity constant is 73.2 pM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules K-562, cellules NIH/3T3, cellules Raji
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HeLa, cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84792-5-RR cible MCM3 dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MCM3 Protéine recombinante Ag7966
• Nom complet: minichromosome maintenance complex component 3
• Masse moléculaire calculée: 91 kDa
• Poids moléculaire observé: 102-105 kDa
• Numéro d’acquisition GenBank: BC001626
• Symbole du gène: MCM3
• Identification du gène (NCBI): 4172
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MCM3, also named as HCC5, P1-MCM3, p102, RLF subunit beta and RLFB, belongs to the MCM family. MCM3 is essential for the initiation of DNA replication and is involved in ensuring that DNA replication is initiated precisely once per cell cycle(PMID: 33093917). The overexpression of MCM3 is related to proliferation of tumor cells(PMID: 31186514).

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