Anticorps Monoclonal anti-MCM4

MCM4 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

2H2A1

N° de cat : 67103-1-Ig

Synonymes

P1 CDC21, hCdc21, EC:3.6.4.12, CDC54, CDC21

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Galerie de données de validation

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  WB analysis using 67103-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67103-1-Ig (MCM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67103-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67103-1-Ig (MCM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67103-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67103-1-Ig (MCM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 67103-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MCM4 antibody (67103-1-Ig, Clone: 2H2A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules HEK-293, cellules HL-60, cellules HSC-T6, cellules Jurkat, cellules NIH/3T3
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67103-1-Ig cible MCM4 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MCM4 Protéine recombinante Ag28642
• Nom complet: minichromosome maintenance complex component 4
• Masse moléculaire calculée: 863 aa, 97 kDa
• Poids moléculaire observé: 97 kDa
• Numéro d’acquisition GenBank: BC031061
• Symbole du gène: MCM4
• Identification du gène (NCBI): 4173
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNA replication licensing factor MCM4 (MCM4), also named Cdc21, acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

Publications

Species	Application	Title
human,mouse	WB	Heliyon; Co-delivery of siRNA and cisplatin via electrospun Nanofibrous membranes for synergistic treatment of malignant melanoma; Authors - Xuewei Zhang

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