Anticorps Monoclonal anti-MCM4

MCM4 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2H2A1

N° de cat : 67103-1-PBS

Synonymes

P1 CDC21, hCdc21, EC:3.6.4.12, CDC54, CDC21

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Galerie de données de validation

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  WB analysis using 67103-1-Ig (same clone as 67103-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67103-1-Ig (MCM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67103-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67103-1-Ig (same clone as 67103-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67103-1-Ig (MCM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67103-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67103-1-Ig (same clone as 67103-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67103-1-Ig (MCM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67103-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 67103-1-Ig (same clone as 67103-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MCM4 antibody (67103-1-Ig, Clone: 2H2A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67103-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67103-1-PBS cible MCM4 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MCM4 Protéine recombinante Ag28642
• Nom complet: minichromosome maintenance complex component 4
• Masse moléculaire calculée: 863 aa, 97 kDa
• Poids moléculaire observé: 97 kDa
• Numéro d’acquisition GenBank: BC031061
• Symbole du gène: MCM4
• Identification du gène (NCBI): 4173
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DNA replication licensing factor MCM4 (MCM4), also named Cdc21, acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

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