Anticorps Polyclonal de lapin anti-MCM5

MCM5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11703-1-PBS

Synonymes

CDC46, EC:3.6.4.12, P1 CDC46, P1-CDC46

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Galerie de données de validation

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  WB analysis using 11703-1-AP (same clone as 11703-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11703-1-AP (MCM5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11703-1-PBS in a different storage buffer formulation.

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  WB analysis using 11703-1-AP (same clone as 11703-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11703-1-AP (MCM5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11703-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 11703-1-AP (same clone as 11703-1-PBS)

  IP result of anti-MCM5 (IP:11703-1-AP, 4ug; Detection:11703-1-AP 1:4000) with Jurkat cells lysate 2400 ug. This data was developed using the same antibody clone with 11703-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse thymus using 11703-1-AP (same clone as 11703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 11703-1-AP (MCM5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11703-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse thymus using 11703-1-AP (same clone as 11703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 11703-1-AP (MCM5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11703-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 11703-1-AP (same clone as 11703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11703-1-AP (MCM5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11703-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 11703-1-AP (same clone as 11703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11703-1-AP (MCM5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11703-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11703-1-AP (same clone as 11703-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCM5 antibody (11703-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11703-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11703-1-PBS cible MCM5 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MCM5 Protéine recombinante Ag2271
• Nom complet: minichromosome maintenance complex component 5
• Masse moléculaire calculée: 82 kDa
• Poids moléculaire observé: 82-88 kDa
• Numéro d’acquisition GenBank: BC000142
• Symbole du gène: MCM5
• Identification du gène (NCBI): 4174
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Minichromosome maintenance, S. Cerevisiae, homolog of 5(MCM5), one component of the complex of MCM2-7, which is the putative replicative essential for once per cell cycle DNA replication initiation and elongation in eukaryotic cells. The MCM complex form a hexameric ring through the interaction surfaces of two neighboring subunits.

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