Anticorps Monoclonal anti-MCM5

MCM5 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

3B7F8

N° de cat : 67049-1-Ig

Synonymes

CDC46, CDC46 homolog, MCM5, P1 CDC46

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Galerie de données de validation

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  WB analysis using 67049-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67049-1-Ig (MCM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 67049-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 67049-1-Ig (MCM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67049-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67049-1-Ig (MCM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 67049-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67049-1-Ig (MCM5 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A375, cellules HeLa, cellules NIH/3T3, cellules RAW 264.7, cellules ROS1728
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

67049-1-Ig cible MCM5 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MCM5 Protéine recombinante Ag28704
• Nom complet: minichromosome maintenance complex component 5
• Masse moléculaire calculée: 82 kDa
• Poids moléculaire observé: 82-88 kDa
• Numéro d’acquisition GenBank: BC000142
• Symbole du gène: MCM5
• Identification du gène (NCBI): 4174
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Minichromosome maintenance, S. Cerevisiae, homolog of 5(MCM5), one component of the complex of MCM2-7, which is the putative replicative essential for once per cell cycle DNA replication initiation and elongation in eukaryotic cells. The MCM complex form a hexameric ring through the interaction surfaces of two neighboring subunits.

Publications

Species	Application	Title
human	IF	Mol Ther; Long noncoding RNA lnc-POP1-1 upregulated by VN1R5 promotes cisplatin resistance in head and neck squamous cell carcinoma through interaction with MCM5.; Authors - Yingying Jiang
mouse	WB	Nat Cancer; Small-molecule inhibitors that disrupt the MTDH-SND1 complex suppress breast cancer progression and metastasis.; Authors - Minhong Shen

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