Anticorps Monoclonal anti-MCM5

MCM5 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3B7F8

N° de cat : 67049-1-PBS

Synonymes

CDC46, CDC46 homolog, MCM5, P1 CDC46

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Galerie de données de validation

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  WB analysis using 67049-1-Ig (same clone as 67049-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67049-1-Ig (MCM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67049-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 67049-1-Ig (same clone as 67049-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 67049-1-Ig (MCM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67049-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67049-1-Ig (same clone as 67049-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67049-1-Ig (MCM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67049-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67049-1-Ig (same clone as 67049-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67049-1-Ig (MCM5 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67049-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67049-1-PBS cible MCM5 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MCM5 Protéine recombinante Ag28704
• Nom complet: minichromosome maintenance complex component 5
• Masse moléculaire calculée: 82 kDa
• Poids moléculaire observé: 82-88 kDa
• Numéro d’acquisition GenBank: BC000142
• Symbole du gène: MCM5
• Identification du gène (NCBI): 4174
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Minichromosome maintenance, S. Cerevisiae, homolog of 5(MCM5), one component of the complex of MCM2-7, which is the putative replicative essential for once per cell cycle DNA replication initiation and elongation in eukaryotic cells. The MCM complex form a hexameric ring through the interaction surfaces of two neighboring subunits.

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