Anticorps Monoclonal anti-MCM7
MCM7 Monoclonal Antibody for IF/ICC
Hôte / Isotype
Mouse / IgG1
Réactivité testée
Humain, rat, souris
Applications
IF/ICC
Conjugaison
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1G1F6
N° de cat : CL488-67446
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en IF/ICC | cellules HepG2, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Informations sur le produit
CL488-67446 cible MCM7 dans les applications de IF/ICC et montre une réactivité avec des échantillons Humain, rat, souris
| Réactivité | Humain, rat, souris |
| Hôte / Isotype | Mouse / IgG1 |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | MCM7 Protéine recombinante Ag28558 |
| Nom complet | minichromosome maintenance complex component 7 |
| Masse moléculaire calculée | 81 kDa |
| Poids moléculaire observé | 85 kDa |
| Numéro d’acquisition GenBank | BC013375 |
| Symbole du gène | MCM7 |
| Identification du gène (NCBI) | 4176 |
| Conjugaison | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Forme | Liquide |
| Méthode de purification | Purification par protéine G |
| Tampon de stockage | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA |
| Conditions de stockage | Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
MCM7, also named as CDC47 homolog or P1.1-MCM3, is a 719 amino acid protein, which belongs to the MCM family. MCM7 acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. the MCM2-7 complex participates in the pre-replication complex formation and exhibits helicase activity which makes DNA unwind, and resulting in recruitment of DNA polymerases and initiation of DNA replication and elongation [PMID: 29463213 ]. An immunohistochemical study has shown that MCM7 is increased in HCC. Silencing of MCM7 with shRNA inhibits the malignant behavior of HCC cells via cell cycle arrest and apoptosis [[PMID: 29463213].
Protocole
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 MCM7 antibody CL488-67446 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
