Anticorps Polyclonal de lapin anti-MCT4

• Phare
• Validé par KD/KO

MCT4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

N° de cat : 22787-1-PBS

Synonymes

MCT-4, SLC16A3, MCT 4, MCT3

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Galerie de données de validation

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  WB analysis of HeLa using 22787-1-AP (same clone as 22787-1-PBS)

  WB result of MCT4 antibody (22787-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCT4 transfected HeLa cells. This data was developed using the same antibody clone with 22787-1-PBS in a different storage buffer formulation.

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  WB analysis using 22787-1-AP (same clone as 22787-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22787-1-AP (MCT4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22787-1-PBS in a different storage buffer formulation.

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  WB analysis using 22787-1-AP (same clone as 22787-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22787-1-AP (MCT4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22787-1-PBS in a different storage buffer formulation.

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  WB analysis of rat skeletal muscle using 22787-1-AP (same clone as 22787-1-PBS)

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22787-1-AP (MCT4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22787-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 22787-1-AP (same clone as 22787-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22787-1-AP (MCT4 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22787-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 22787-1-AP (same clone as 22787-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22787-1-AP (MCT4 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22787-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 22787-1-AP (same clone as 22787-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 22787-1-AP (MCT4 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22787-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 22787-1-AP (same clone as 22787-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22787-1-AP (MCT4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22787-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 22787-1-AP (same clone as 22787-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22787-1-AP (MCT4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22787-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 22787-1-AP (same clone as 22787-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22787-1-AP (MCT4 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22787-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 22787-1-AP (same clone as 22787-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22787-1-AP (MCT4 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22787-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 22787-1-AP (same clone as 22787-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22787-1-AP (MCT4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22787-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 22787-1-AP (same clone as 22787-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22787-1-AP (MCT4 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22787-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 22787-1-AP (same clone as 22787-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 22787-1-AP (MCT4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22787-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 22787-1-AP (same clone as 22787-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 22787-1-AP (MCT4 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22787-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 22787-1-AP (same clone as 22787-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22787-1-AP (MCT4 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22787-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 22787-1-AP (same clone as 22787-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22787-1-AP (MCT4 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22787-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 22787-1-AP (same clone as 22787-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MCT4 antibody (22787-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 22787-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 22787-1-AP (same clone as 22787-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MCT4 antibody (22787-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 22787-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 22787-1-AP (same clone as 22787-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MCT4 antibody (22787-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 22787-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 22787-1-AP (same clone as 22787-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.8 ug Anti-Human MCT4 (22787-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 22787-1-PBS in a different storage buffer formulation.

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Informations sur le produit

22787-1-PBS cible MCT4 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MCT4 Protéine recombinante Ag18788
• Nom complet: solute carrier family 16, member 3 (monocarboxylic acid transporter 4)
• Masse moléculaire calculée: 465 aa, 49 kDa
• Poids moléculaire observé: 38-42 kDa
• Numéro d’acquisition GenBank: BC112269
• Symbole du gène: MCT4
• Identification du gène (NCBI): 9123
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The monocarboxylate transporter 4 (MCT4, also known as SLC16A3) is involved in the transportation of metabolically important monocarboxylates such as lactate, pyruvate, acetate and ketone bodies. It is widely expressed, particularly strongly in glycolytic tissues such as white skeletal muscle fibres, astrocytes, white blood cells, chondrocytes and some mammalian cell lines. MCT4 is also linked to tumor biology because it mediates lactate transport across membranes resulting in antiapoptotic effects.

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