Anticorps Polyclonal de lapin anti-MDC1

MDC1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24721-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 24721-1-AP (same clone as 24721-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24721-1-AP (MDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24721-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 24721-1-AP (same clone as 24721-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24721-1-AP (MDC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24721-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 24721-1-AP (same clone as 24721-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24721-1-AP (MDC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24721-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 24721-1-AP (same clone as 24721-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MDC1 antibody (24721-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 24721-1-PBS in a different storage buffer formulation.

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Informations sur le produit

24721-1-PBS cible MDC1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MDC1 Protéine recombinante Ag20135
• Nom complet: mediator of DNA damage checkpoint 1
• Masse moléculaire calculée: 2089 aa, 227 kDa
• Numéro d’acquisition GenBank: BC152556
• Symbole du gène: MDC1
• Identification du gène (NCBI): 9656
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MDC1, also named as KIAA0170 or NFBD1, is a 2089 amino acid protein, which contains two BRCT domains and one FHA domain. MDC1 localizes in the nucleus and is highly expressed in testis. MDC1 is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. MDC1 may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked.

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