Anticorps Recombinant de lapin anti-MDC1

MDC1 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240469D5

N° de cat : 83496-1-RR

Synonymes

NFBD1, NFBD 1, MDC 1, 240469D5

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Galerie de données de validation

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  WB analysis of human testis using 83496-1-RR

  human testis tissue were subjected to SDS PAGE followed by western blot with 83496-1-RR (MDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83496-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83496-1-RR (MDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 83496-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MDC1 antibody (83496-1-RR, Clone: 240469D5 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 83496-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83496-1-RR against Human MDC1 were performed. The affinity constant is 0.346 nM.

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain, cellules HEK-293, cellules HeLa, cellules K-562, cellules MCF-7, cellules PC-3
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83496-1-RR cible MDC1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MDC1 Protéine recombinante Ag20135
• Nom complet: mediator of DNA damage checkpoint 1
• Masse moléculaire calculée: 2089 aa, 227 kDa
• Poids moléculaire observé: 260-320 kDa
• Numéro d’acquisition GenBank: BC152556
• Symbole du gène: MDC1
• Identification du gène (NCBI): 9656
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MDC1, also named as KIAA0170 or NFBD1, is a 2089 amino acid protein, which contains two BRCT domains and one FHA domain. MDC1 localizes in the nucleus and is highly expressed in testis. MDC1 is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. MDC1 may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked.

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