Anticorps Monoclonal anti-MDH1

• Phare
• Validé par KD/KO

MDH1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F9A2

N° de cat : 66505-1-PBS

Synonymes

MDHA, KAR, EC:1.1.1.96, EC:1.1.1.37, Aromatic alpha-keto acid reductase

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Galerie de données de validation

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  WB analysis using 66505-1-Ig (same clone as 66505-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66505-1-Ig (MDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66505-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66505-1-Ig (same clone as 66505-1-PBS)

  WB result of MDH1 antibody (66505-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MDH1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66505-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66505-1-Ig (same clone as 66505-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66505-1-Ig (MDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66505-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66505-1-Ig (same clone as 66505-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66505-1-Ig (MDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66505-1-PBS in a different storage buffer formulation.

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  IHC staining of rat heart using 66505-1-Ig (same clone as 66505-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66505-1-Ig (MDH1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66505-1-PBS in a different storage buffer formulation.

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  IHC staining of rat heart using 66505-1-Ig (same clone as 66505-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66505-1-Ig (MDH1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66505-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66505-1-Ig (same clone as 66505-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66505-1-Ig (MDH1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66505-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 66505-1-Ig (same clone as 66505-1-PBS)

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human MDH1 (66505-1-Ig, Clone:1F9A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66505-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66505-1-PBS cible MDH1 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MDH1 Protéine recombinante Ag8744
• Nom complet: malate dehydrogenase 1, NAD (soluble)
• Masse moléculaire calculée: 334 aa, 36 kDa
• Poids moléculaire observé: 36-37 kDa
• Numéro d’acquisition GenBank: BC001484
• Symbole du gène: MDH1
• Identification du gène (NCBI): 4190
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MDH1 (Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme. (PMID:10693743)

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